knockout

standard Loss of Function Mutations – Approaches, Limitations

Technologies are democratizing insect genetics and it seems as if everyone is thinking about making ‘knockouts’.  This is a good thing but as Housden et al. nicely explain in a recent paper in Nature Reveiws Genetics explain: “although LOF (loss […]

vasa Cas9 expressing Drosophila. From Xue et al. 2014

standard Vasa Cas9 Experession Yields Good Mutation Rates in Drosophila

Xue and colleagues report efficient knock-out and knock-in manipulations of the Drosophila genome with a Cas9 transgenic system. Others have made similar observations.  Xue et al. report the germline mutational rate ranging from 14% to 95%. They also demonstrated the […]