knock-out

standard High Knock in/out Rates in Aedes aegypti Expressing Cas9 from a Transgene.

  Li et al have published some interesting data on the preprint server bioRΧiv regarding the creation and use of Aedes aegypti lines expressing an SpCas9 transgene in the germline.  The report substantial improvements in gRNA-mediated mutagenesis rates. Cas9 mediated […]

Synthetic gene drive system based on site specific DNA endonucleases (homing endonucleases, ZFN, TALENS, Cas9). Target sites and homology arms are chosen so that the endonuclease and associated genes (gRNA and other transgenes) are copied into homologous chromosomes.

standard An Overview of Gene Drive

Gantz and Bier reflect on and consider the implications of Cas9-based genome editing on genetics in a recent Bioessays that is worth reading if you are trying to keep up on this rapidly evolving part of the genetics ecosystem. Not […]

standard Editing Tribolium’s Genome

Gilles et al (2015) recently published a paper in Development describing CRISPR-mediated gene targeting and gene replacement in the flour beetle Tribolium castaneum. The authors started with the Tribolium line Pig-19, which strongly expresses enhanced green fluorescent protein (EGFP) in […]

standard Plasmids for Rapid and Versatile Genome Editing

As is the case for many other organisms, CRISPR has rapidly become the method of choice for targeted genome modification in insects. Much of the method development has been taking place in the model organism Drosophila melanogaster, where, in little […]