injection

Electroporation Theory

standard Injectoporation for Gene Delivery

Let’s see.  What do we have so far? Great genomic resources?  Check. Great gene vectors?  Check. Great genome modification technologies?  Check. Effective insect genetic technology delivery systems for somatic and germ cells?  Not so much, unfortunately. Gene, transgene and RNA […]

standard φC31 in Anopheles gambiae: A How-To

In terms of genetic engineering of insects, the current most commonly used technique for germline transformation involves the use of mobile transposable genetic elements, which are simply inefficient, have limited carrying capacities, and integrate randomly into genomes resulting in insertional […]

Genetic Technology Delivery by Embryo Microinjection

standard Robotic Injection of Embryos – Ready for Insects?

  Spaink et al (2013) describe their use of a robotic injection system as a high throughput screening tool in zebra fish, Danio rerio, embryos.  Automated and semi-automated injection systems for zebra fish embryos as well as other systems are […]

Platynereis dumerilii adult

standard Genetically Modified Annelids: Platynerieis dumerilii. Not Unlike Modifying Insects

The bristle worm, Platynerieis dumerilii, is a marine annelid with a biology that makes it of interest because of the development of its nervous system, reproductive cycles that are synchronized with lunar phases and its neuroendocrinology among other things. Even […]

Culex quinquifasciatus pupa

standard dsRNA into Pupae for Adult Silencing – Mosquitoes

Although injection of large volumes and amounts of dsRNA into insects is somewhat of a blunt instrument for performing reverse genetics, it has been used with great success in many systems while in others it has been less useful. Stage […]