gRNA

standard Dominant Maternal Effects Caused by CRISPR/Cas9

Expressing Cas9 and guide RNAs from transgenes integrated into the genome of organisms typically leads to higher rates of mutagenesis of the target gene compared to when those components are provided transiently by microinjection, for example. Lin and Potter describe […]

Cas9 + sgRNA

standard CRISPRscan – A Tool Aiding Selection of CRISPR-Cas9 gRNAs

Just published online in Nature Methods, Moreno-Mateos et al report testing the activities of >1200 gRNAs in zebrafish embryos and used the results to create an algorithm for predicting gRNA efficacy. The advent of the CRISPR-Cas9 system has revolutionized genome […]

standard Editing Tribolium’s Genome

Gilles et al (2015) recently published a paper in Development describing CRISPR-mediated gene targeting and gene replacement in the flour beetle Tribolium castaneum. The authors started with the Tribolium line Pig-19, which strongly expresses enhanced green fluorescent protein (EGFP) in […]

Basics of Cas9 Activity

standard Cas9 Gene Drive & Classical Biological Control

Webber et al have published an Opinion essay in PNAS concerning the growing interest in using engineered endonucleases (Cas9 in particular) to create gene drive systems to combat invasive species. Homing endonucleases are well known and well studied site-specific endonucleases […]

standard CRISPR with Independent Transgenes or Mutagenic Chain Reaction for Creating Homozygotes in Lab?

In fall of 2014, two publications came from George Church’s group (Esvelt et al., 2014; Oye et al., 2014) postulating on the potential for the CRISPR/Cas9 system to be used as an autonomous gene drive for various pest management goals. […]

standard Comparing TALE- and dCas9-Based Designer Transcription Factors

Genome editing with programmable nucleases using transcription activator-like effector nucleases (TALENs) or RNA-guided clustered regularly interspersed short palindromic repeat (CRISPR) associated protein (Cas9) have become popular and important technologies. These systems can be modified to carry other functional protein domains […]

Cas9 + sgRNA

standard Purified Cas9 Protein for Insect Genome Modification with CRISPRs

The interest in CRISPR/Cas9 genome-editing and -modification technologies is quite extensive and there has been a flood of data dealing with optimizing efficiency among other things.               In insect systems there are a number […]