germline

standard Western Corn Rootworm, Diabrotica virgifera virgifera, germline transformation.

Chu and colleagues in a recently published paper in Insect Molecular Biology describe their successful efforts to deploy transposon-based technologies in the economically important beetle, Diabrotica virgifera virgifera, and it is an interesting case study in how to develop genetic […]

standard Megaselia and Chironomus Germline Transformation

In a recent paper in Development Genes Evolution., Caroti et al. (2015) reported on their efforts to extend the piggyBac transposon-based germline transformation system to the Diptera Megaselia abdita and Chironomus riparius and to use it for in vivo labelling […]

Anopheles stephensi Image from: Vectorbase

standard Towards a Medea Drive System in Anopheles Mosquitoes

Germ-line specific promoters are useful to insect biologists and Biedler and colleagues (2015) in a just-published paper in G3-Genes|Genomes|Genetics report their efforts to identify, isolate and test maternal germline-specific genes and promoters in the Asian malaria vector, Anopheles stephensi. “We […]

standard Two Bombyx Testis-Specific Promoters

The level of sophistication with which one can genetically manipulate insects is very much determined by how well transgene expression can be regulated. Xu et al (2014) in Insect Molecular Biology describe their successful efforts to isolate promoters from two […]