standard Microinjection independent genome engineering

Takahashi et al. (2105) describe a microinjection independent genome engineering method for mice that is an interesting innovation that seeks to address technical challenges associated with the creation of transgenic and genetically modified mice that have good analogs in insect […]

Electroporation Theory

standard Electroporating the CRISPR/Cas9 System

Qin et al. (2015) describe their successful efforts to use electroporation technology to introduce CRISPR/Cas9 system components into mouse embryos and while not exactly insect biology their motivations for pursuing this approach are shared by many insect biologists with an interest […]

Electroporation Theory

standard Electroporation of Horn Fly Embryos

Qiang Xu, et al. 2015 describe their efforts to transform the horn fly, Haematobia irritans, by elctroporation. Transgenic technology has been considered an effective technique for the construction of genetically-modified insects for the purposes of insect control. Microinjection with piggyBac transposable […]

Electroporation Theory

standard Injectoporation for Gene Delivery

Let’s see.  What do we have so far? Great genomic resources?  Check. Great gene vectors?  Check. Great genome modification technologies?  Check. Effective insect genetic technology delivery systems for somatic and germ cells?  Not so much, unfortunately. Gene, transgene and RNA […]

Basic structure of a morpholino oligonucleotide

standard Have You Considered Morpholinos?

Many are making efforts to move genetic technologies into insect systems where they have not been used before  and becoming painfully aware that some technologies may not be applicable to all insects. For example, dsRNA may work well when systemically […]