editing

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standard Gene Editing Spodoptera

Three papers recently described successful CRISPR/Cas9 mutagenesis in two species of Spodoptera.  Spodoptera litura and S. littoralis. Bi et al (2016) use Cas9 to create somatic mutations in S. litura in developmental gene abdominal-A, abd-A, and analyzed the resulting larvae […]

standard Phosphorothioate oligomers increase knock-in frequency and precision in TALEN- and Cas9-mediated genome editing

In a recent paper by Renaud et al. (2016), the authors investigate methods to increase genome editing knock-in efficiency by modifying the chemistry of the donor oligomers to be resistant to nuclease activity. The modifications they tested included locked nucleic […]

standard Dopamine receptor knock-outs in crickets

Awata et al. (2015) have used Cas9 to knock-out Dop1 in Gryllus bimaculatus to assess the role of dopamine neurons in mediating reward and punishment. Functional genomics technologies like transposons and first and second generation gene-editing technologies have been available […]

"Papilio machaon MHNT CUT 2013 3 11 Cahors Female" by Didier Descouens - Own work. Licensed under CC BY-SA 4.0 via Commons - https://commons.wikimedia.org/wiki/File:Papilio_machaon_MHNT_CUT_2013_3_11_Cahors_Female.jpg#/media/File:Papilio_machaon_MHNT_CUT_2013_3_11_Cahors_Female.jpg

standard CRISPR/Cas9 Gene Editing in Papilionid Butterflies

Li et al (2015) recently published an exciting paper on butterflies genomics and functional genomics . Li et al have worked towards producing high quality genomes for extremely heterozygous and closely related butterflies P. xuthus and P. machaon. Further, they […]

Structure of a TALE binding to DNA

standard Optimized TALEN Application and Screening in Drosophila melanogaster

Lee et al 2015 provide a detailed methodology for gene editing using TALENs in Drosophila melanogaster. While TALEN use is relatively well established in D. melanogaster, fine tuning the injection concentrations of designer nucleases allows for the maximum efficiency in […]

Electroporation Theory

standard Electroporating the CRISPR/Cas9 System

Qin et al. (2015) describe their successful efforts to use electroporation technology to introduce CRISPR/Cas9 system components into mouse embryos and while not exactly insect biology their motivations for pursuing this approach are shared by many insect biologists with an interest […]

standard Pulling Recent CRISPR/Cas9 Work in Drosophila Together

Xu et al. review the recent history of using CRISPR/Cas9-based technologies in Drosophila melanogaster. Within the last two years there have been numerous publications describing the use of CRISPR/Cas9-based technologies in Drosophila melanogaster.  These last two years have been a time […]

Cas9 + sgRNA

standard Purified Cas9 Protein for Insect Genome Modification with CRISPRs

The interest in CRISPR/Cas9 genome-editing and -modification technologies is quite extensive and there has been a flood of data dealing with optimizing efficiency among other things.               In insect systems there are a number […]

BuDN (Nuclease) and TALENs (from Stella et al. 2014)

standard BuD: Another Tunable DNA-Binding Domain for Genome Modification

Tunable DNA-binding domains with high specificity remain important tools for constructing genome modifications even with the current popularity of the CRISPR/Cas9 system. Stella at al (2014) describe their analysis of a DNA-binding protein, BurrH, from the symbiotic bacterium Burkholderia rhizoxinica […]

TALENs and CRISPRs

standard Multigene Mutagenesis of Silk Moth with CRISPRs.

Genome editing using CRISPR/Cas9 is changing the way we think about genome modification. The CRISPR system, because it relies on a small user-provide guide RNA (gRNA) to give the Cas9 endonuclease its sequence specificity, is easy to use, relative to, […]

I-CreI Crystal Structure

standard Delivering Reprogramed Meganucleases With Lentiviruses- Is this Applicable to “Difficult” Insects?

Delivering biologically relevant nucleic acids and proteins to appropriate cells of multicellular organisms for the purposes of genetically modifying either somatic or germline genomes is a central problem for not only insect biologists but for those working on other organisms […]

Platynereis dumerilii adult

standard Genetically Modified Annelids: Platynerieis dumerilii. Not Unlike Modifying Insects

The bristle worm, Platynerieis dumerilii, is a marine annelid with a biology that makes it of interest because of the development of its nervous system, reproductive cycles that are synchronized with lunar phases and its neuroendocrinology among other things. Even […]

standard Development, Growth & Differentiation Devote Issue to Genome Editing

Genome editing with engineered nucleases is an emerging technology that enables manipulation of targeted genes in many organisms and cell lines. Development, Growth & Differentiation has devoted an entire issue to genome editing.  The paper describing the successful use of […]