dsRNA

Simplified Illustration of RNAi

standard Short vs. Long Hairpin RNAs for RNAi?

Bartoletti and colleagues recently published a paper in Genesis: The Journal of Genetics and Development that reports short hairpin RNAs being more effective than long hairpins in causing loss-of-function phenotypes of pointed (Pnt)  in Drosophila melanogaster. RNA interference (RNAi) has been used […]

standard Cas9 Mutagenesis in Houseflies Confirms Male Determining Factor Identification

Sharma et al. (2107) report in a recent issue of Science the identification of a male determining factor in the housefly Musca domestica and provide convincing reverse genetic confirmatory evidence using dsRNA-mediated gene silencing and Cas9-mediated mutagenesis. Alternative splicing of […]

Boll Weevil, Anthonomous grandis.

standard Ribonucleoprotein Particles Improve Oral RNAi Delivery to Crop Pests

The efficacy of RNAi in insects depends in part on efficient delivery and uptake of dsRNA.  Gillet et al. (2017) report in Frontiers of Physiology on their use of protein-complexed dsRNA to silence genes in Anthonomous grandis following oral delivery, […]

standard Genetic Dissection of RNAi in the Colorado Potato Beetle, Leptinotarsa decemlineata

RNAi works well in beetles, in general, and why that is is not all that clear but Yoon et al. (2016) have begun to identify key genetic contributors in their recent paper in Insect Biochemistry and Molecular Biology.  The authors […]

on. Image credit: www.biocomicals.com by Alper Uzun, PhD

standard RNAi in Beetles and Leps – Why the Difference?

It is well established that the RNAi response of Coleoptera and Lepidoptera differ greatly but less is known about the basis for these differences.  Skukla et al (2016) systematically investigated this question and report their insights in a recent paper […]

standard Post-feeding and -injection Dynamics of dsRNA in 4 Insects

The variability of dsRNA-induced RNA interference is well documented with some insects very amenable to this method of regulating gene expression while are others are very much less so. The factors involved in this variation are known to some extent […]

standard Lipoplexes and oral dsRNA delivery to Blattella germanica

Lin et al., in Pesticide Management Science have described their efforts to orally deliver dsRNA to the German cockroach, Blattella germanica. Blattella germanica is fairly sensitive to RNAi when dsRNA is injected into the hemocoel.  Directly feeding B. germanica dsRNA […]

standard Carbon Quantum Dots for dsRNA Delivery

Das et al. (2015) in Applied Materials & Interfaces compare chitosan, silica and carbon quantum dot nanoparticles as dsRNA delivery platforms for silencing genes in larval mosquitoes. Direct injection of dsRNA into the hemocoel of insects has  been somewhat effective […]

standard Extreme Transgenerational Gene Silencing

Abdellatef et al. (2015) recently published the results of their efforts to silence the salivary sheath protein gene in the aphid Sitobion avenae in the Plant Biotechnology Journal and observed what might be called extreme transgenerational gene silencing. Plant-expressed dsRNA […]

standard Parental RNAi Via Oral Delivery

Khajuria et al (2015) have published a short paper in Insect Biochemistry and Molecular Biology describing their observations supporting the idea that Diabrotica vigifera exhibits parental RNAi following the oral delivery of dsRNA. Parental RNAi (pRNAi) refers to an inter-generational […]

RNAi as an insecticide.

standard Insecticidal miRNA: Alternative to dsRNA and siRNA

RNA interference (RNAi) technology has been widely proposed to control insect pests by targeting their vital genes. Small interfering RNA (siRNA)-assisted RNAi has been reported in several previous studies. However, Agrawal et al 2015 report microRNA-assisted plant-mediated RNAi to control […]

standard Plastid Mediated RNAi: A Novel Insecticidal Tool

Recently, Zhang et al demonstrated a robust and promising way of controlling the devastating solanaceous crops insect pest, Colorado potato beetle (Leptinotarsa decemlineata; CPB), using a modified strategy for delivering plant mediated RNAi. Increasing insect herbivores’ resistance to plant defense mechanisms […]

Basic structure of a morpholino oligonucleotide

standard Have You Considered Morpholinos?

Many are making efforts to move genetic technologies into insect systems where they have not been used before  and becoming painfully aware that some technologies may not be applicable to all insects. For example, dsRNA may work well when systemically […]

Feeding Pea Aphid, Acyrthosiphon pisum

standard RNA Silencing in Plants to Fight Aphids

Aphids feed from the plant phloem sap and are responsible for large damage on crops by either weakening the plant or transmitting plant viruses that circulate in the sap. Can we use transgenic plants to limit aphid negative impact on […]

Culex quinquifasciatus pupa

standard dsRNA into Pupae for Adult Silencing – Mosquitoes

Although injection of large volumes and amounts of dsRNA into insects is somewhat of a blunt instrument for performing reverse genetics, it has been used with great success in many systems while in others it has been less useful. Stage […]

standard Feed or Inject dsRNA in Aphids – It Depends

RNAi-based gene silencing has been reported but it has been problematic. The authors’ direct comparison of injection and feeding of dsRNA targeting the cathepsin-L gene in the pea aphid, Acyrthosiphon pisum. Single aphids were analyzed at the level of five […]

Feeding or Microinjecting dsRNA into Aphids for Gene Silencing.

standard Aphid Hemolymph and Gut Degrade dsRNA

Aphids are sap-sucking insects of great economic importance, and recently the pea aphid Acyrthosiphon pisum had its genome sequenced and annotated.  Insect genetic technologies are in high demand for this insect but it is proving to be a real challenge. […]