Drosophila

Drosophila melanogaster

standard Cas9 Expression Patterns Impact on Resistance Evolution in Different Genetic Backgrounds

Gene drive systems are sensitive to the evolution of resistance in the form of polymorphisms in the guide RNA’s target DNA sequence.  Champer et al. (2017) have recently reported on their exploration of how Cas9 expression patterns as well as […]

standard Dominant Maternal Effects Caused by CRISPR/Cas9

Expressing Cas9 and guide RNAs from transgenes integrated into the genome of organisms typically leads to higher rates of mutagenesis of the target gene compared to when those components are provided transiently by microinjection, for example. Lin and Potter describe […]

Adult male spotted wing drosophila, Drosophilia suzukii (Matsumura). Photograph by Martin Hauser, California Department of Food and Agriculture.

standard Gene Editing in an Invasive Species

Li and Scott report their successful use of Cas9 to knockout two genes in Drosophila suzukii in a recent issue of Biochemical and Biophysical Research Communications. Drosophila suzukii or the spotted wing Drosophila although a native of Asia has recently […]

Synthetic gene drive system based on site specific DNA endonucleases (homing endonucleases, ZFN, TALENS, Cas9). Target sites and homology arms are chosen so that the endonuclease and associated genes (gRNA and other transgenes) are copied into homologous chromosomes.

standard An Overview of Gene Drive

Gantz and Bier reflect on and consider the implications of Cas9-based genome editing on genetics in a recent Bioessays that is worth reading if you are trying to keep up on this rapidly evolving part of the genetics ecosystem. Not […]

standard Genetic Manipulation of Insect Cell Lines

Two papers in the December issue of Genetics, Cherbas et al. 2015 and Manivannan et al. 2015, describe an improved method for making Drosophila cell lines with single, targeted mutations. Both works build upon existing recombination-mediated cassette exchange (RMCE) technology […]

standard Cas9-Mediated Gene-Drive In Mosquitoes

Gantz and Bier who demonstrated the fundamental principles of a Cas9-based gene drive system using Drosophila melanogaster in a publication earlier this year have recreated that system in the mosquito Anopheles stephensi with the help of collaborators.  Gantz et al. […]

standard Editing Tribolium’s Genome

Gilles et al (2015) recently published a paper in Development describing CRISPR-mediated gene targeting and gene replacement in the flour beetle Tribolium castaneum. The authors started with the Tribolium line Pig-19, which strongly expresses enhanced green fluorescent protein (EGFP) in […]

Structure of a TALE binding to DNA

standard Optimized TALEN Application and Screening in Drosophila melanogaster

Lee et al 2015 provide a detailed methodology for gene editing using TALENs in Drosophila melanogaster. While TALEN use is relatively well established in D. melanogaster, fine tuning the injection concentrations of designer nucleases allows for the maximum efficiency in […]

standard Short Course on Insect Genetic Technologies – August 2015

The Insect Genetic Technologies Research Coordination Network is offering a short course on insect genetic technologies August 17-21, 2015. Today there are myriad technologies for modifying genomes. Technologies are emerging rapidly and it is a challenge for investigators to keep […]

standard Exchangeable Cassettes Controlled by Endogenous Genes

Two recent publications, Gnerer et al. and Diao et al., address the issue of recreating the fine transcriptional patterns of endogenous genes with binary expression systems in insects. Binary expression systems are extremely powerful tools for controlled gene manipulation. The […]

standard Pulling Recent CRISPR/Cas9 Work in Drosophila Together

Xu et al. review the recent history of using CRISPR/Cas9-based technologies in Drosophila melanogaster. Within the last two years there have been numerous publications describing the use of CRISPR/Cas9-based technologies in Drosophila melanogaster.  These last two years have been a time […]

standard New ΦC31 Resources

A rapid method for generating and finding ΦC31 landing sites (attP) with the desired expression characteristics as well as a new method for creating transgene arrays in vivo was recently described by Knapp et al (2015). Albeit these methods were […]

FlyFacility, Cambridge University. A insect genetic technologies service provider and resource

standard The Fly Facility at the University of Cambridge Department of Genetics

The Department of Genetics at the University of Cambridge is primarily located in a large Edwardian building close to the centre of town. The department has a long and distinguished history of Drosophila research beginning with John Thoday’s evolutionary genetics […]

standard The Drosophila RNAi Screening Center (DRSC) – A functional genomics resource useful to all insect biologists.

The Drosophila RNAi Screening Center (DRSC) at Harvard Medical School was founded by Prof. Norbert Perrimon in 2003 to provide community support for genome-wide RNAi screens in cultured Drosophila cells. Now, more than a decade later, we are still going […]

Anopheles stephensi Image from: Vectorbase

standard Towards a Medea Drive System in Anopheles Mosquitoes

Germ-line specific promoters are useful to insect biologists and Biedler and colleagues (2015) in a just-published paper in G3-Genes|Genomes|Genetics report their efforts to identify, isolate and test maternal germline-specific genes and promoters in the Asian malaria vector, Anopheles stephensi. “We […]

standard Plasmids for Rapid and Versatile Genome Editing

As is the case for many other organisms, CRISPR has rapidly become the method of choice for targeted genome modification in insects. Much of the method development has been taking place in the model organism Drosophila melanogaster, where, in little […]

standard A Collection of Methods for Editing Insect Genomes

The August 2014 issue of METHODS is devoted to the topic of “Editing and Investigating Genomes with TALE and CRISPER/Cas Systems: Genome Engineering Across Species Using TALENS” Five of the 14 protocols included in this issue are specifically focused on […]

standard Q System: Flies, Worms – Now Fish

“Transgenic tools for manipulation of gene expression are invaluable for labeling and tracking cell populations and for assessing genetic and cellular functions in developmental, physiological and behavioral studies.” (Subedi et al. 2014) The Gal4/UAS system is the most widely used […]

Cas9 + sgRNA

standard Purified Cas9 Protein for Insect Genome Modification with CRISPRs

The interest in CRISPR/Cas9 genome-editing and -modification technologies is quite extensive and there has been a flood of data dealing with optimizing efficiency among other things.               In insect systems there are a number […]

standard Compendium of Fly Methods – Good for Other Insect Biologists

Elsevier has recently published a volume of Methods dedicated to Drosophila developmental biology tools.  These include general tools such a forward genetic tools as well as genome engineering tools among others. Another section focuses on tools and methods for studying […]

standard Mutagenesis, Transposons and Transgenes: State-of-the-Art

The genetic tools available for use in Drosophila melanogaster are second to none. Insect scientists working on other insect systems and who also want to acquire or develop various genetic technologies should always be keeping their eyes on technological developments […]

standard Six Tandem Cherries -> Very Bright Insects

Insect genetic modification technologies rely heavily on the use of fluorescent proteins as genetic markers, expression reporters etc. Colors vary, as do fluorescent outputs. Shearin et al. (2014) describe their efforts to increase signals from two popular fluorescent proteins, EGFP […]

TALENs and CRISPRs

standard Multigene Mutagenesis of Silk Moth with CRISPRs.

Genome editing using CRISPR/Cas9 is changing the way we think about genome modification. The CRISPR system, because it relies on a small user-provide guide RNA (gRNA) to give the Cas9 endonuclease its sequence specificity, is easy to use, relative to, […]

Chimera, a mythic creature composed of lion, snake and goat

standard Marking Clones: Past and Future

As Perrimon and colleagues point out chimeras or mosaics have played and continue to play an important role in our analysis of development.  In Drosophilia the technology for creating mosaics is mature and powerful.  Even before transgenic technologies provided precision […]

standard Improved Lineage Marking in Drosophila

Marking cells has been a powerful tool for developmental biologists studying Drosophila melanogaster for many decades.  Insect genetic technologies, in particular site specific recombination systems, have allowed this lineage marking and tracking method to become highly robust and controlable.  Enhancer […]

standard Development, Growth & Differentiation Devote Issue to Genome Editing

Genome editing with engineered nucleases is an emerging technology that enables manipulation of targeted genes in many organisms and cell lines. Development, Growth & Differentiation has devoted an entire issue to genome editing.  The paper describing the successful use of […]

Uses for nuclease defective CRISPRS

standard CRISPR/Cas9 in Drosophila – Other Insects not far behind.

While genome editing technologies have been available for the past decade or so, until recently they have been challenging and expensive to use. The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR/Cas9) system is changing all of that. This bacterial defense […]