Dicer

Simplified Illustration of RNAi

standard Short vs. Long Hairpin RNAs for RNAi?

Bartoletti and colleagues recently published a paper in Genesis: The Journal of Genetics and Development that reports short hairpin RNAs being more effective than long hairpins in causing loss-of-function phenotypes of pointed (Pnt)  in Drosophila melanogaster. RNA interference (RNAi) has been used […]

standard Plastid Mediated RNAi: A Novel Insecticidal Tool

Recently, Zhang et al demonstrated a robust and promising way of controlling the devastating solanaceous crops insect pest, Colorado potato beetle (Leptinotarsa decemlineata; CPB), using a modified strategy for delivering plant mediated RNAi. Increasing insect herbivores’ resistance to plant defense mechanisms […]

standard Tripodal RNA: An Enhanced RNAi Trigger

RNA interference-based manipulation of gene expression is a highly versatile tool available to insect scientists.  In some cases direct injection of large amounts of long dsRNA will suffice.  In other cases, not so much.  Combining siRNA with some kind of […]