Bombyx

standard Glycoengineering Silkworms Using Transposon Technology

Mabashi-Asazuma et al. (2015)  report in Insect Biochemistry and Molecular Biology on their successful efforts to alter post translational glycolytic processing of proteins in the posterior silk gland of Bombyx mori. The “silkworm is emerging as a new recombinant protein […]

Bombyx mori with eggs

standard A Stable, Replaceable, Highly Efficient Transgene Expression System

Long et. al. in Scientific Reports describe a system for creating stable sites in the genome of Bombyx mori that allow for site-specific recombination-mediated integration of transgenes. Transposons are essential platforms for many insect genetic technologies and are typically used […]

standard Two Bombyx Testis-Specific Promoters

The level of sophistication with which one can genetically manipulate insects is very much determined by how well transgene expression can be regulated. Xu et al (2014) in Insect Molecular Biology describe their successful efforts to isolate promoters from two […]

standard Transgenic Effectors for Neurogenic Manipulation

Genetic technologies will continue to play an increasingly important role in enabling the function of specific neurons in insect behavior to be determined. The genetic technologies available for manipulating Drosophila melanogaster provides not only great examples but some of these […]

standard A Collection of Methods for Editing Insect Genomes

The August 2014 issue of METHODS is devoted to the topic of “Editing and Investigating Genomes with TALE and CRISPER/Cas Systems: Genome Engineering Across Species Using TALENS” Five of the 14 protocols included in this issue are specifically focused on […]

Bombyx mori with eggs

standard Transgenic TALEN Expression to Control Sex in Bombyx

Analysis of gene function in insects by editing the genome, or reverse genetics, is now greatly facilitated thanks to powerful genetic technologies, such as TALENs, ZFNs, and more recently CRISPR/Cas9, becoming available in many organisms. However, screening of transgenic mutated […]

Bombyx mori

standard Strongly and Ubiquitously Expressed Promoter for Lepidoptera

The number of genetic technologies available for the study of the silkworm, Bombyx mori, is second only to those available for Drosophila melanogaster. Germ-line transformation, enhancer-trapping, genome editing/modification using CRISPRs, TALENS, ZFNs  are all established technologies in B. mori. But, […]

TALENs and CRISPRs

standard Multigene Mutagenesis of Silk Moth with CRISPRs.

Genome editing using CRISPR/Cas9 is changing the way we think about genome modification. The CRISPR system, because it relies on a small user-provide guide RNA (gRNA) to give the Cas9 endonuclease its sequence specificity, is easy to use, relative to, […]

GAL4+ Oenocytes

standard Oenocyte-specific GAL4 Expression in Bombyx

GAL4 is a well-established binary transcription system that can provide a level of modularity to expressing transgenes in specific patterns.  Tsubota et al describe the construction of GAL4 transgenes under the regulatory control of promoters from genes with strong expressing […]