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standard Genetic Manipulation of Insect Cell Lines

Two papers in the December issue of Genetics, Cherbas et al. 2015 and Manivannan et al. 2015, describe an improved method for making Drosophila cell lines with single, targeted mutations. Both works build upon existing recombination-mediated cassette exchange (RMCE) technology […]

standard Anopheles gambiae Transgenesis Tool Box – Major Additions

Volohonsky et. al. (2015) in a just-published paper in G3 entitled  “Tools for Anopheles gambiae transgenesis”  describe a wealth of new tools for creating transgenic An. gambiae. There has been a protocol for Anopheles gambiae transgenesis for over 15 years, […]

Strategy for Y engineering; from Bernardini et al. 2014

standard Advanced Mosquito Genetic Engineering

For mosquitoes the number of genetic modification technologies that can and have been applied continues to grow, and while still not as deep as the Drosophila toolbox the mosquito toolbox is still impressive in the right hands. Bernardini et al. […]