aphid

Feeding Pea Aphid, Acyrthosiphon pisum

standard RNA Silencing in Plants to Fight Aphids

Aphids feed from the plant phloem sap and are responsible for large damage on crops by either weakening the plant or transmitting plant viruses that circulate in the sap. Can we use transgenic plants to limit aphid negative impact on […]

standard Feed or Inject dsRNA in Aphids – It Depends

RNAi-based gene silencing has been reported but it has been problematic. The authors’ direct comparison of injection and feeding of dsRNA targeting the cathepsin-L gene in the pea aphid, Acyrthosiphon pisum. Single aphids were analyzed at the level of five […]

EMS-induced Aphid Mutants.

standard Aphid Genetics

Reverse genetics has become a particularly popular approach to the genetic analysis of insects because of the widespread  applicability of gene silencing technologies like the injection or feeding of dsRNA.  While a relatively blunt tool, dsRNA injection has been used […]

Feeding or Microinjecting dsRNA into Aphids for Gene Silencing.

standard Aphid Hemolymph and Gut Degrade dsRNA

Aphids are sap-sucking insects of great economic importance, and recently the pea aphid Acyrthosiphon pisum had its genome sequenced and annotated.  Insect genetic technologies are in high demand for this insect but it is proving to be a real challenge. […]