Aedes

Aedes aegypti, vector of Dengue virus

standard Cre/lox-Recombinase-Mediated Cassette Exchange in Aedes aegypti

Häcker et al 2017 reports the use of Cre/lox Recombination-Mediated Cassette Exchange (RMCE) in Aedes aegypti providing a pathway for interrogating specific genomic locations for the use of transgenic constructs in future genetic control methods. Häcker et al 2017 first generated […]

standard Finding Enhancers in Mosquitoes and Beyond

This post was written by Joseph Sarro and Keshava Mysore In a recent paper published in BMC Genomics, Behura and Sarro et al. (2016) report on the use of FAIRE-seq, formaldehyde-assisted isolation of regulatory elements paired with DNA sequencing, for […]

standard Carbon Quantum Dots for dsRNA Delivery

Das et al. (2015) in Applied Materials & Interfaces compare chitosan, silica and carbon quantum dot nanoparticles as dsRNA delivery platforms for silencing genes in larval mosquitoes. Direct injection of dsRNA into the hemocoel of insects has  been somewhat effective […]

standard New ΦC31 Resources

A rapid method for generating and finding ΦC31 landing sites (attP) with the desired expression characteristics as well as a new method for creating transgene arrays in vivo was recently described by Knapp et al (2015). Albeit these methods were […]

standard A Collection of Methods for Editing Insect Genomes

The August 2014 issue of METHODS is devoted to the topic of “Editing and Investigating Genomes with TALE and CRISPER/Cas Systems: Genome Engineering Across Species Using TALENS” Five of the 14 protocols included in this issue are specifically focused on […]

Everyone against Dengue

standard How to Release Genetically Modified Mosquitoes – The Right Way

Insect genetic technologies are enabling researchers to conceive of new ways to control insects and to improve on established control methods. In mosquitoes there have been a number of ideas for using genetic technologies to reduce mosquito populations or to […]