CRISPR/Cas Web Resources

Web Resources for design and planning of CRISPR/Cas9 studies

from Bassett and Liu (2014) CRISPR/Cas9 and Genome Editing in Drosophila. J. Genetics and Genomics 41, 7-19 doi.10.1016/j.gg.2013.12.004

Name Link Brief description Reference
Drosophila CRISPR web resources
OxfCRISPR (Liu Lab) http://www.oxfcrispr.org/ Oxford Fly CRISPR Resources Bassett et al., 2013
CRISPRflydesign (Bullock Lab) http://www.crisprflydesign.org/ Offers Cas9 transgenic stocks n/a
DRSC CRISPR finder (Perrimon Lab) http://www.flyrnai.org/crispr/ A web tool to identify CRISPRs for fly study Ren et al., 2013
FlyCas9 (Ueda Lab) http://www.shigen.nig.ac.jp/fly/nigfly/cas9/index.jsp Provides reagents, protocols and online tools for genome engineering by the designer nuclease Cas9 in Drosophila Kondo and Ueda, 2013
flyCRISPR (O’Connor-Giles Lab, Wildonger Lab and Harrison Lab) http://flycrispr.molbio.wisc.edu/ Fly CRISPR resources Gratz et al., 2013
flyCRISPR discussion group https://groups-google-com.proxy-um.researchport.umd.edu/forum/#!forum/flycrispr-discussion-group A forum for sharing developments, insights, ideas and asking questions related to fly CRISPRs Gratz et al., 2013
Fly target sites (Zhang Lab) http://www.genome-engineering.org/crispr/?page_id=41 For application of Cas9 for site-specific genome editing in eukaryotic cells and organisms Hsu et al., 2013
General CRISPR resources
Addgene CRISPR plasmids http://www.addgene.org/CRISPR/ A collection of CRISPR plasmids and reagents n/a
Crass: The CRISPR Assembler http://ctskennerton.github.io/crass/ A program that searches through raw metagenomic reads for CRISPRs Skennerton et al., 2013
CRISPI http://crispi.genouest.org/ A web interface with graphical tools and functions allows users to find CRISPR in personal sequences. Rousseau et al., 2009
CRISPR Discussion Forum https://groups-google-com.proxy-um.researchport.umd.edu/forum/#!forum/crispr A forum to discuss Genome Engineering using CRISPR/Cas systems Cong et al., 2013
CRISPRmap http://rna.informatik.uni-freiburg.de/CRISPRmap Web server provides an automated assignment of newly sequenced CRISPRs to standard classification system Lange et al., 2013
CRISPRs web server http://crispr.u-psud.fr/ A gateway to publicly accessible CRISPRs database and software including CRISPRFinder, CRISPRdb and CRISPRcompar Grissa et al., 2007a, Grissa et al., 2007b and Grissa et al., 2008
CRISPRTarget http://bioanalysis.otago.ac.nz/CRISPRTarget Predicts the most likely targets of CRISPR RNAs Biswas et al., 2013
E-CRISP http://www.e-crisp.org/ A software tool to design and evaluate CRISPR target sites n/a
Goldstein Lab CRISPR http://wormcas9hr.weebly.com/ A genome engineering resource for the Caenorhabditis elegans research community Dickinson et al., 2013
Joung Lab CRISPR http://www.crispr-cas.org/ A genome engineering resource for zebrafish research community Hwang et al., 2013
Zhang Lab Genome Engineering http://www.genome-engineering.org/ CRISPR genome engineering resources website Cong et al., 2013
ZiFiT target design tool http://zifit.partners.org/ZiFiT/ Identifies potential target sites in DNA sequences Sander et al., 2007 and Sander et al., 2010
n/a, not available.