Germ-line Expression of Cas9 Improves Knock-in Rates

Optimization of genome editing is a moving target but Gratz et al. find that germ-line specific expression of Cas9 yielded the highest frequency of knock-in events in Drosophila melanogaster. This approach could be taken in a number of other insect species for which the creation of transgenics with germ-line specific Cas9 expression is feasible.

 

Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue LK, Harrison MM, Wildonger J, O’Connor-Giles KM: Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease. Genetics 2013, 194:1029-+. 10.1534/genetics.113.152710

 

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