Li et al. recently reported how systemic RNAi occurred in the western corn rootworm (WCR), Diabrotica virgifera virgifera, one of the most important maize pests in North America and a model for understanding the mechanism of RNAi. Efficiency of RNA […]
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Redkmer – a computational tool to identify X-linked targets for CRISPR-based sex distortion in insects
In the inaugural volume of The CRISPR Journal, Philippos Papathanos and Nikolai Windbichler describe a computational pipeline they call redkmer, which identifies repetitive CRIPSR target sites for X-shredding systems in insects. Back in 2007, Windbichler et al. found that the […]
A recent review by Darrington et al. (2017) sets the stage for a suite of novel RNAi-based approaches to help extend the applicability of SIT to many more pest species. The sterile insect technique (SIT) has been successfully used for […]
In the January 2018 issue of Pathogens and Global Health Adelman et al. seek to establish standard operating procedures (SOPs) for gene drive research in arthropods and ask fundamental questions as to why these procedures are necessary. At a time […]
In their recent paper in G3: Genes|Genomes|Genetics, Li et al. describe a highly efficient protocol for the generation of stable knockout lines using CRISPR/Cas9. The ability of effectively targeting and disrupting gene function is essential for gene functional analysis studies […]
The Journal of Responsible Innovation has published a special issue entitled: Roadmap to Gene Drives: Research and Governance Needs in Social, Political, and Ecological Context. This is a collection of 13 papers and is the outcome of workshop held in […]
Four recently published papers on containment issues concerning genetically modified insects. 1. Recommendations for Laboratory Containment and Management of Gene Drive Systems in Arthropods. Benedict Mark Q., Burt Austin, Capurro Margareth L., De Barro Paul, Handler Alfred M., Hayes Keith […]
Li and Handler (2017) have recently reported using CRISPR/Cas9 to make a temperature-sensitive transformer-2 (tra-2) allele in the pest species Drosophila suzukii by mutating the nucleotides orthologous to the Drosophila melanogaster tra-2ts2 allele. This study demonstrates an important proof of […]
Advances in CRISPR/Cas9 Genome Editing in Drosophila Lead the Way for Applications in Other Insects.
In the January edition of FlyBook (Genetics) Bier et al. review the recent advances in CRISPR/Cas technologies applied to the fruit fly, delving into the details of established and cutting-edge methods and their potential applications to future studies in this […]
2018 IGTRCN Technical Short Course on Insect Genetic Technologies: Theory and Practice July 22 – 27, 2018 The Insect Transformation Facility Institute for Bioscience and Biotechnology Research University of Maryland College Park Applications by April 2, 2018 (24 seats available) […]