In a recent paper in Genome Research Nakajima et al. 2018 propose an efficient gene editing strategy by nucleotide substitution that overcomes three general problems associated with current methods. First, indels caused by the traditional Cas9-induced Double Strand Breaks (DSB), […]
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Komor et al. reviewed two strategies for genome editing that do not rely on the Double Strand Breaks (DSB) in a recent issue ACS Chemical Biology. The authors specifically address oligonucleotide-directed mutagenesis and base pairing. Possibly the most harmful type […]
In a recent study using zebrafish published in G3: Genes, Genomes, Genetics, Hu et al. (2018) conducted a comprehensive analysis of gene editing efficiency between Cas9 proteins and Cas9 mRNA, each fused with a nuclear-localization signal (NLS) in a different […]
In a recent report in PNAS, Buchman et al. (2018) describe the construction and development of a MEDEA-based gene drive system in the crop pest, Drosophila suzukii. Buchman et al revisited the MEDEA-based gene drive approach developed in D. melanogaster […]
In their recent review in Trends in Biotechnology, Zhang et al 2017 provide an overview of how the RNAi pathway can be utilized to control pests, how different insect pests provide unique challenges to overcome and how those challenges can […]
Recently, Stroble et al. (2018) published in the journal, eLIFE, a novel technique to quickly and efficiently develop homozygous transgenic lines. Although this method was tested in the genetic model organism, Tribolium castaneum, it could be of particular interest for […]
Roggenkamp et al 2018, in a recent publication in G3:Genes, Genomes, Genetics provide an in-depth analysis of potential ways that gene drive mechanisms can be regulated by targeting four conserved elements which would be found in any CRISPR/Cas9 based gene […]
Dong et al. (2018) in a recent paper in PLOS Pathogens describe the results of the genetic inactivation of a broad-spectrum agonist of the malaria parasite, FREP1 (fibrinogen-related protein 1, member of an immune and pattern recognition gene family called […]
Marshall and Akbari review a range of different proposed gene drive systems and discuss ways in which CRISPR may be useful in engineering them in a recent issue of ACS Chemical Biology. In the years since translocations were first suggested […]
A recent issue of ACS Chemical Biology includes some 25 articles on various aspects of CRISPR/Cas systems – mechanisms and applications. While not all of the articles will be of direct interest to insect biologists, many are and anyone working […]