Technology Topics Submission

Enhancing Homology Repair-Mediated Genome Editing

In a recent paper in Nature Biotechnology, Richardson et al (2016) report on the interaction of target-site bound Cas9 and dCas9 on DNA strand accessibility- and have identified an optimized approach to increase homologous recombination (HR) mediated repair. They found […]

Phosphorothioate oligomers increase knock-in frequency and precision in TALEN- and Cas9-mediated genome editing

In a recent paper by Renaud et al. (2016), the authors investigate methods to increase genome editing knock-in efficiency by modifying the chemistry of the donor oligomers to be resistant to nuclease activity. The modifications they tested included locked nucleic […]

Finding Enhancers in Mosquitoes and Beyond

This post was written by Joseph Sarro and Keshava Mysore In a recent paper published in BMC Genomics, Behura and Sarro et al. (2016) report on the use of FAIRE-seq, formaldehyde-assisted isolation of regulatory elements paired with DNA sequencing, for […]

Guide RNA interacting with target DNA. Image credit: William Reid

Intramolecular interactions in gRNA may influence Cas9 cleavage activity

In an article recently published in Nature Communications, Thyme et al (2016) discuss the interactions of the sequences of sgRNAs, the role of chromatin, and how these factors might interfere with Cas9 cleavage activity. By investigating the activity of Cas9 […]

CRISPR-SD, a programmable Cas9 approach to generating X-shredder lines in insects with XY mating systems

In a recent paper in Scientific Reports, Galizi, Hammond, et al report on the use of CRISPR-Cas9 to develop a sex-distortion system in Anopheles gambiae, which they termed CRISPRSD. Earlier, the group had demonstrated that transgenic An. gambaie expressing the […]