William Reid

Redkmer – a computational tool to identify X-linked targets for CRISPR-based sex distortion in insects

In the inaugural volume of The CRISPR Journal, Philippos Papathanos and Nikolai Windbichler describe a computational pipeline they call redkmer, which identifies repetitive CRIPSR target sites for X-shredding systems in insects. Back in 2007, Windbichler et al. found that the […]

CRISPR-SD, a programmable Cas9 approach to generating X-shredder lines in insects with XY mating systems

In a recent paper in Scientific Reports, Galizi, Hammond, et al report on the use of CRISPR-Cas9 to develop a sex-distortion system in Anopheles gambiae, which they termed CRISPRSD. Earlier, the group had demonstrated that transgenic An. gambaie expressing the […]

Phosphorothioate oligomers increase knock-in frequency and precision in TALEN- and Cas9-mediated genome editing

In a recent paper by Renaud et al. (2016), the authors investigate methods to increase genome editing knock-in efficiency by modifying the chemistry of the donor oligomers to be resistant to nuclease activity. The modifications they tested included locked nucleic […]

Enhancing Homology Repair-Mediated Genome Editing

In a recent paper in Nature Biotechnology, Richardson et al (2016) report on the interaction of target-site bound Cas9 and dCas9 on DNA strand accessibility- and have identified an optimized approach to increase homologous recombination (HR) mediated repair. They found […]

CRISPR-Cas9 mediated “Mutagenic Chain Reaction”

A recent paper in Science by Gantz and Bier (2015) investigated the potential of utilizing the CRISPR-Cas9 system as, essentially, a programmable homing endonuclease to generate homozygous loss-of-function (LOF) mutants. Advances in genome editing have allowed for the directed generation of […]