David O'Brochta

standard Trojan Female Technique: Mitochondria-based population suppression.

The ‘Trojan Female Technique” is a genetics-based population suppression strategy.  Wolff et al.(2017) report results of empirical tests of the idea using Drosophila melanogaster as a model system and show net population suppression. Genetics-based pest control strategies are becoming increasingly […]

Cas9 with functional domains highlighted in relation to target DNA and guideRNA

standard RAD51 and Valporic Acid Enhance Cas9-induced HR

Takayama et al (2017) describe in Nucleic Acids Research how they increased the frequency of biallelic editing of genes in human ES/iPS cells and their findings may be useful to insect biologists. The efficiency of Cas9-mediated homologous recombination (HR) can […]

standard Cas9 Mutagenesis in Houseflies Confirms Male Determining Factor Identification

Sharma et al. (2107) report in a recent issue of Science the identification of a male determining factor in the housefly Musca domestica and provide convincing reverse genetic confirmatory evidence using dsRNA-mediated gene silencing and Cas9-mediated mutagenesis. Alternative splicing of […]

Boll Weevil, Anthonomous grandis.

standard Ribonucleoprotein Particles Improve Oral RNAi Delivery to Crop Pests

The efficacy of RNAi in insects depends in part on efficient delivery and uptake of dsRNA.  Gillet et al. (2017) report in Frontiers of Physiology on their use of protein-complexed dsRNA to silence genes in Anthonomous grandis following oral delivery, […]

standard Western Corn Rootworm, Diabrotica virgifera virgifera, germline transformation.

Chu and colleagues in a recently published paper in Insect Molecular Biology describe their successful efforts to deploy transposon-based technologies in the economically important beetle, Diabrotica virgifera virgifera, and it is an interesting case study in how to develop genetic […]