David O'Brochta

Underdominance. Heterozygotes are less fit than either possible homozygotes. 01 vs 00 or 11

standard Conditions for Successful Underdominance Gene Drive

Edgington and Alphey (2017) report their modeling efforts in the Journal of Theoretical Biology on conditions under which engineered underdominance gene drive systems could result in successful population replacement. Underdominance refers to a situation where heterozygotes are selected against within […]

standard High Knock in/out Rates in Aedes aegypti Expressing Cas9 from a Transgene.

  Li et al have published some interesting data on the preprint server bioRΧiv regarding the creation and use of Aedes aegypti lines expressing an SpCas9 transgene in the germline.  The report substantial improvements in gRNA-mediated mutagenesis rates. Cas9 mediated […]

Drosophila melanogaster

standard Cas9 Expression Patterns Impact on Resistance Evolution in Different Genetic Backgrounds

Gene drive systems are sensitive to the evolution of resistance in the form of polymorphisms in the guide RNA’s target DNA sequence.  Champer et al. (2017) have recently reported on their exploration of how Cas9 expression patterns as well as […]

standard Trojan Female Technique: Mitochondria-based population suppression.

The ‘Trojan Female Technique” is a genetics-based population suppression strategy.  Wolff et al.(2017) report results of empirical tests of the idea using Drosophila melanogaster as a model system and show net population suppression. Genetics-based pest control strategies are becoming increasingly […]