vasa Cas9 expressing Drosophila. From Xue et al. 2014

Vasa Cas9 Experession Yields Good Mutation Rates in Drosophila

Xue and colleagues report efficient knock-out and knock-in manipulations of the Drosophila genome with a Cas9 transgenic system. Others have made similar observations.  Xue et al. report the germline mutational rate ranging from 14% to 95%. They also demonstrated the […]

Feed or Inject dsRNA in Aphids – It Depends

RNAi-based gene silencing has been reported but it has been problematic. The authors’ direct comparison of injection and feeding of dsRNA targeting the cathepsin-L gene in the pea aphid, Acyrthosiphon pisum. Single aphids were analyzed at the level of five […]

Strategy for Y engineering; from Bernardini et al. 2014

Advanced Mosquito Genetic Engineering

For mosquitoes the number of genetic modification technologies that can and have been applied continues to grow, and while still not as deep as the Drosophila toolbox the mosquito toolbox is still impressive in the right hands. Bernardini et al. […]

Basic structure of a morpholino oligonucleotide

Morpholino use in Mosquitoes is Promising

Antisense technologies have had limited application because oligodexoynucleotides in vivo have very limited stability. This stability problem due to rapid degradation of short DNA molecules limits the bioavailability of the molecules. Over the years there has been an evolution of […]

Daphnia pulex

Water Fleas and Insects – Common Challenges for Genetic Modification – Shared Solutions?

The water flea, Daphnia pulex, is a microcrustacean that has become a model for biomedical research and, perhaps more significantly, a model for probing G x E (Genetics x Environment) interactions. Some genetic modification methods have been developed for Daphnia […]

Apis mellifera

Transgenic Honeybees – Finally!

Genome modification technologies have been increasing steadily in recent years. With the exception of direct genome editing technologies (ZFNs, TALENs and CRISPRs) variously technologies for use in insects rely heavily on transposon platforms to move these technologies into target genomes. […]

Arthropod Genomics Symposium

Arthropod Genomics Symposium + IGTRCN in 2015 !

The Arthropod Genomics Symposium continues to be a popular and very useful meeting for those interested in insect genomics and its application to a wide array of biological problems. The program is always well constructed, the speakers excellent, and the […]