Technology Topics

Bombyx mori

standard Promoter activities in transgenic insects.

Ye et al. in a just published paper in Scientific Reports address some questions regarding transgene expression in Bombyx mori that are likely to be of interest to other working with transgenic insects. The relatively random patterns of transposable element […]

Untitled

standard FISSEQ – in situ RNAseq

Lee et al. (2015) describe an exciting new method in Nature Protocols  that combines the benefits of in situ hybridization with RNA-Seq. They named the method FISSEQ, for fluorescent in situ sequencing of RNA. Many researchers, including myself, would like […]

BIOhazard

standard New Genetic Containment Technologies

Mandell and colleagues (2015), in a very recent paper in Nature, describe their successful efforts to take  the concept of synthetic biology and applied it to biocontainment of bacteria (E. coli), building in synthetic amino acids as safeguards should they […]

phiC31

standard New ΦC31 Resources

A rapid method for generating and finding ΦC31 landing sites (attP) with the desired expression characteristics as well as a new method for creating transgene arrays in vivo was recently described by Knapp et al (2015). Albeit these methods were […]

Electroporation Theory

standard Electroporation of Horn Fly Embryos

Qiang Xu, et al. 2015 describe their efforts to transform the horn fly, Haematobia irritans, by elctroporation. Transgenic technology has been considered an effective technique for the construction of genetically-modified insects for the purposes of insect control. Microinjection with piggyBac transposable […]

Network Announcements

Coleomegilla_maculata_02

standard Lady Beetle Pigmentation Mutants Available

I have selected a stable strain of pigmentation defective lady beetles, species Coleomegilla maculata. They are missing the red pigment in both cuticle and eyes. These insects are available for research purposes. Please contact me for information.     Authors […]

IGTRCN Trainees 2014

standard IGTRCN Trainees – Fall 2014!

The IGTRCN is working to diffuse knowledge and technology from those who ‘know’ and ‘have’ to those who do not but need to. The Peer-to-Peer Training Fellowships are intended to facilitate the process by providing modest resources to encourage interactions. […]

Positions Available

standard Mobile Laboratory Instructor

Mobile Laboratory Instructor Position at MdBio Foundation MdBio Foundation is looking to an enthusiastic individual to teach and manage laboratory activities for Maryland high school students on our mobile laboratory, MdBioLab.  Vacancy is immediate. Above all, we are looking for […]

Screen-Shot-2014-11-25-at-4.32.02-PM

standard Postdoctoral Associate, Cornell University

POSITION RESPONSIBILITIES: Molecular, proteomic and functional analysis of Ae. aegypti seminal fluid proteins. These efforts include cloning, transient knockdown of genes of interest and functional studies. In addition to carrying out research central to this NIH project, and obtaining publishable […]

Knowledgebase

FlyFacility, Cambridge University.  A insect genetic technologies service provider and resource

standard Fly Facility, University of Cambridge, UK

Insect Genetic Technologies Service Provider – Drosophila – Non-commercial Fly Facility, University of Cambridge, UK – http://www.flyfacility.gen.cam.ac.uk Our Drosophila microinjection service is available to fly researchers throughout the European Union. We can inject flies (including non-melanogaster Drosophila species) for P-element/piggyback […]

Service Providers

standard Insect Genetic Technologies Service Providers

Insect Genetic Technology Service Providers If you would like to add to this list use the “Make a Submission” button and select “Knowledgebase” For insects other than Drosophila melanogaster Non-Commercial The Institute for Bioscience and Biotechnology Insect Transformation Facility, University […]

CRISPRWebResources

standard CRISPR/Cas Web Resources

Web Resources for design and planning of CRISPR/Cas9 studies from Bassett and Liu (2014) CRISPR/Cas9 and Genome Editing in Drosophila. J. Genetics and Genomics 41, 7-19 doi.10.1016/j.gg.2013.12.004 Name Link Brief description Reference Drosophila CRISPR web resources OxfCRISPR (Liu Lab) http://www.oxfcrispr.org/ […]

CRISPRsDrosophila

standard CRISPR In Drosophila

Comparison of CRISPR genome engineering studies in Drosophila from Bassett and Liu (2014) CRISPR/Cas9 and Genome Editing in Drosophila. J. Genetics and Genomics 41, 7-19 doi.10.1016/j.gg.2013.12.004 Reference Gratz et al., 2013 Bassett et al., 2013 Yu et al., 2013 Kondo and Ueda, 2013 […]