Technology Topics

Tcastaneum

standard Editing Tribolium’s Genome

Gilles et al (2015) recently published a paper in Development describing CRISPR-mediated gene targeting and gene replacement in the flour beetle Tribolium castaneum. The authors started with the Tribolium line Pig-19, which strongly expresses enhanced green fluorescent protein (EGFP) in […]

Cas9 with functional domains highlighted in relation to target DNA and guideRNA

standard CRISPR-STAT to Evaluate Cas9/guideRNAs

Carrington et al (2015) describe another approach to functionally assessing guide RNAs for use with Cas9  that is quick, relatively easy and inexpensive. After designing and assembling guide RNAs to be used to direct Cas9 to specific targets, investigators would […]

CasActivator

standard Mis-Expressing Insect Genes Using Cas9

Lin et al. have now published a paper that demonstrates overexpression of endogenous genes in Drosophila melanogaster using the CRISPR/Cas system. Over- and miss-expression of genes can be a powerful tool to elucidate gene function and is commonly achieved through […]

Cas9

standard Cas9 Gene Drive & Classical Biological Control

Webber et al have published an Opinion essay in PNAS concerning the growing interest in using engineered endonucleases (Cas9 in particular) to create gene drive systems to combat invasive species. Homing endonucleases are well known and well studied site-specific endonucleases […]

Network Announcements

EntomopathoCongress

standard 5th International Entomopathogens and Microbial Control Congress

“The Fifth International Participated Entomopathogens and Microbial Control Congress” Ankara, Turkey   September 9-11, 2015. Entomopathogens form an interesting area of research in pest management as being one of the most important tools of environmetally-friendly, applicable and effective pest management strategies […]

Positions Available

Tribolium_castaneum

standard CRISPR Beetles!

If you enjoy the challenge of developing CRISPR as a new technology for the control of stored product beetles, I need you! ARS Postdoc open immediately in my laboratory: https://www.usajobs.gov/GetJob/ViewDetails/410850300 Questions? Email me: bso@ksu.edu Thanks, Brenda Oppert

standard PostDoctoral Position: Insect Symbiosis/Microbial Ecology

POST-DOCTORAL POSITION: Insect symbiosis/Microbial ecology A multidisciplinary research team working on “Multi- and Interdisciplinary Approaches to Understand Spatio-Temporal Patterns of Insect Pests and Design Landscapes for Sustainable Pest Management in Tropical Agricultural Systems” is seeking for an independent, self-motivated post-doc. […]

isu

standard Post-doc position in Mosquito Immunity

The Smith Lab in the Department of Entomology at Iowa State University is recruiting a talented individual to investigate how the innate immune response limits malaria parasites in the mosquito host. Recent work has demonstrated that mosquito blood cells (or […]

Knowledgebase

FlyFacility, Cambridge University.  A insect genetic technologies service provider and resource

standard Fly Facility, University of Cambridge, UK

Insect Genetic Technologies Service Provider – Drosophila – Non-commercial Fly Facility, University of Cambridge, UK – http://www.flyfacility.gen.cam.ac.uk Our Drosophila microinjection service is available to fly researchers throughout the European Union. We can inject flies (including non-melanogaster Drosophila species) for P-element/piggyback […]

Service Providers

standard Insect Genetic Technologies Service Providers

Insect Genetic Technology Service Providers If you would like to add to this list use the “Make a Submission” button and select “Knowledgebase” For insects other than Drosophila melanogaster Non-Commercial The Institute for Bioscience and Biotechnology Insect Transformation Facility, University […]

CRISPRWebResources

standard CRISPR/Cas Web Resources

Web Resources for design and planning of CRISPR/Cas9 studies from Bassett and Liu (2014) CRISPR/Cas9 and Genome Editing in Drosophila. J. Genetics and Genomics 41, 7-19 doi.10.1016/j.gg.2013.12.004 Name Link Brief description Reference Drosophila CRISPR web resources OxfCRISPR (Liu Lab) http://www.oxfcrispr.org/ […]

CRISPRsDrosophila

standard CRISPR In Drosophila

Comparison of CRISPR genome engineering studies in Drosophila from Bassett and Liu (2014) CRISPR/Cas9 and Genome Editing in Drosophila. J. Genetics and Genomics 41, 7-19 doi.10.1016/j.gg.2013.12.004 Reference Gratz et al., 2013 Bassett et al., 2013 Yu et al., 2013 Kondo and Ueda, 2013 […]