Technology Topics

TIDE

standard Hi, Tide.

The use of sequence-targeting nucleases (TALENs, CRISPR-Cas9, zinc finger nucleases) to introduce small insertions/deletions into the genome of an organism is an effective means of generating specific mutants. Although these technologies are effective, they still require testing and optimization to [...]

FlyRNAismall

standard RNAi Screening

RNAi continues to be a very important tool for performing genetic analyses. It is particularly attractive because it can be used in many insect systems. However, it works better in some than in others. Of course, this technology has been [...]

Cas9 with functional domains highlighted in relation to target DNA and guideRNA

standard CRISPR/Cas9 for RNA Cleavage

The CRISPR/Cas9 system is quickly becoming the most versatile method for modifying DNA targets, but researchers seeking equivalent control against RNA transcripts have been left behind. In a new paper by O’Connell et. al, (2014) the authors describe a clever [...]

Network Announcements

Flybase

standard FlyBase Helps Launch IGTRCN

FlyBase is “A Database of Drosophila Genes & Genomes” sent email notifications of the IGTRCN’s launch to its subscribers.  The success of the IGTRCN will depend very much on tapping into the technologies and technological knowledge within the fly community. [...]

ESA Logo

standard IGTRCN in Entomology Today

Entomology Today is blog feature of the Entomological Society of America that features current and interesting news relevant to the insect science community. The ESA was helpful in launching IGTRCN.org marking the IGTRCN ‘going public’. ESA’s cooperation and support are [...]

Peer-to-Peer Training Fellowships.

standard Fellowships Available!

Application Deadline: October 1, 2014 The IGTRCN announces its first Request For Applications for Peer-to-Peer Training Fellowships. IGTRCN Peer-to-Peer Training Fellowships are intended to support knowledge and technology development and dissemination among insect science communities. Fellowships will consist of up [...]

Activities

Knowledgebase

Service Providers

standard Insect Genetic Technologies Service Providers

Insect Genetic Technology Service Providers If you would like to add to this list use the “Make a Submission” button and select “Knowledgebase” For insects other than Drosophila melanogaster Non-Commercial The Institute for Bioscience and Biotechnology Insect Transformation Facility, University [...]

CRISPRWebResources

standard CRISPR/Cas Web Resources

Web Resources for design and planning of CRISPR/Cas9 studies from Bassett and Liu (2014) CRISPR/Cas9 and Genome Editing in Drosophila. J. Genetics and Genomics 41, 7-19 doi.10.1016/j.gg.2013.12.004 Name Link Brief description Reference Drosophila CRISPR web resources OxfCRISPR (Liu Lab) http://www.oxfcrispr.org/ [...]

CRISPRsDrosophila

standard CRISPR In Drosophila

Comparison of CRISPR genome engineering studies in Drosophila from Bassett and Liu (2014) CRISPR/Cas9 and Genome Editing in Drosophila. J. Genetics and Genomics 41, 7-19 doi.10.1016/j.gg.2013.12.004 Reference Gratz et al., 2013 Bassett et al., 2013 Yu et al., 2013 Kondo and Ueda, 2013 [...]

220px-Hessian_Fly

standard Hessian Fly Polytene FISH

Cytogenetic analysis using FISH in combination with polytene chromosome preparations is a powerful tool. Characterization of genetically modified insects can benefit greatly from this type of analysis. Here is a protocol used for Hessian Fly Polytene FISH analysis provided by [...]