Technology Topics

RNAi

standard Issues in RNAi Delivery and Development

RNA silencing has  human therapeutic potential and there is a large research enterprise focused on this topic addressing problems of efficacy and delivery – topics of great interest to insect biologist looking to exploit the power of gene silencing for [...]

editing

standard Comparing TALE- and dCas9-Based Designer Transcription Factors

Genome editing with programmable nucleases using transcription activator-like effector nucleases (TALENs) or RNA-guided clustered regularly interspersed short palindromic repeat (CRISPR) associated protein (Cas9) have become popular and important technologies. These systems can be modified to carry other functional protein domains [...]

tripodal RNA

standard Tripodal RNA: An Enhanced RNAi Trigger

RNA interference-based manipulation of gene expression is a highly versatile tool available to insect scientists.  In some cases direct injection of large amounts of long dsRNA will suffice.  In other cases, not so much.  Combining siRNA with some kind of [...]

IGTRCN Trainees 2014

standard IGTRCN Trainees – Fall 2014!

The IGTRCN is working to diffuse knowledge and technology from those who ‘know’ and ‘have’ to those who do not but need to. The Peer-to-Peer Training Fellowships are intended to facilitate the process by providing modest resources to encourage interactions. [...]

Network Announcements

IGTRCN Trainees 2014

standard IGTRCN Trainees – Fall 2014!

The IGTRCN is working to diffuse knowledge and technology from those who ‘know’ and ‘have’ to those who do not but need to. The Peer-to-Peer Training Fellowships are intended to facilitate the process by providing modest resources to encourage interactions. [...]

Flybase

standard FlyBase Helps Launch IGTRCN

FlyBase is “A Database of Drosophila Genes & Genomes” sent email notifications of the IGTRCN’s launch to its subscribers.  The success of the IGTRCN will depend very much on tapping into the technologies and technological knowledge within the fly community. [...]

ESA Logo

standard IGTRCN in Entomology Today

Entomology Today is blog feature of the Entomological Society of America that features current and interesting news relevant to the insect science community. The ESA was helpful in launching IGTRCN.org marking the IGTRCN ‘going public’. ESA’s cooperation and support are [...]

Peer-to-Peer Training Fellowships.

standard Fellowships Available!

Application Deadline: October 1, 2014 The IGTRCN announces its first Request For Applications for Peer-to-Peer Training Fellowships. IGTRCN Peer-to-Peer Training Fellowships are intended to support knowledge and technology development and dissemination among insect science communities. Fellowships will consist of up [...]

Positions Available

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standard Postdoctoral Associate, Cornell University

POSITION RESPONSIBILITIES: Molecular, proteomic and functional analysis of Ae. aegypti seminal fluid proteins. These efforts include cloning, transient knockdown of genes of interest and functional studies. In addition to carrying out research central to this NIH project, and obtaining publishable [...]

Knowledgebase

Service Providers

standard Insect Genetic Technologies Service Providers

Insect Genetic Technology Service Providers If you would like to add to this list use the “Make a Submission” button and select “Knowledgebase” For insects other than Drosophila melanogaster Non-Commercial The Institute for Bioscience and Biotechnology Insect Transformation Facility, University [...]

CRISPRWebResources

standard CRISPR/Cas Web Resources

Web Resources for design and planning of CRISPR/Cas9 studies from Bassett and Liu (2014) CRISPR/Cas9 and Genome Editing in Drosophila. J. Genetics and Genomics 41, 7-19 doi.10.1016/j.gg.2013.12.004 Name Link Brief description Reference Drosophila CRISPR web resources OxfCRISPR (Liu Lab) http://www.oxfcrispr.org/ [...]

CRISPRsDrosophila

standard CRISPR In Drosophila

Comparison of CRISPR genome engineering studies in Drosophila from Bassett and Liu (2014) CRISPR/Cas9 and Genome Editing in Drosophila. J. Genetics and Genomics 41, 7-19 doi.10.1016/j.gg.2013.12.004 Reference Gratz et al., 2013 Bassett et al., 2013 Yu et al., 2013 Kondo and Ueda, 2013 [...]

220px-Hessian_Fly

standard Hessian Fly Polytene FISH

Cytogenetic analysis using FISH in combination with polytene chromosome preparations is a powerful tool. Characterization of genetically modified insects can benefit greatly from this type of analysis. Here is a protocol used for Hessian Fly Polytene FISH analysis provided by [...]