Technology Topics

Danaus plexippus      By William Warby (https://flic.kr/p/dtKZF2) [CC BY 2.0 (http://creativecommons.org/licenses/by/2.0)], via Wikimedia Commons

standard Monarch Reverse Genetics

Markert et al. just reported their effective use Cas9 and TALENs to knock out cryptochrome 2 and clock in Danaus plexippus using some strategies and approaches that are generally useful to insect scientists and much more efficient than earlier reports […]

ProNuclearInjection

standard Animal and Insect Transgenics – Parallels

James Murray and Elizabeth Maga recently recounted briefly the development and evolution of animal transgenic technologies in a recent issue of Transgenic Research and the similarities and differences between the development and evolution of insect transgenic technologies, which were ongoing […]

Adult male spotted wing drosophila, Drosophilia suzukii (Matsumura). Photograph by Martin Hauser, California Department of Food and Agriculture.

standard Gene Editing in an Invasive Species

Li and Scott report their successful use of Cas9 to knockout two genes in Drosophila suzukii in a recent issue of Biochemical and Biophysical Research Communications. Drosophila suzukii or the spotted wing Drosophila although a native of Asia has recently […]

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standard Enhancing Homology Repair-Mediated Genome Editing

In a recent paper in Nature Biotechnology, Richardson et al (2016) report on the interaction of target-site bound Cas9 and dCas9 on DNA strand accessibility- and have identified an optimized approach to increase homologous recombination (HR) mediated repair. They found […]

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standard Another High-Fidelity SpCas9 Nuclease

Kleinstiver et al. describe in Nature their successful efforts to create high-fidelity SpCas9 nuclease variants that have high on-target efficiencies and low or no off-target effects.  The results are SpCas9-HF1. When using Cas9 or other engineered nuclease systems one hopes […]

Network Announcements

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standard 2016 IGTRCN Technical Short Course – APPLY NOW!

2016 IGTRCN Technical Short Course on Insect Genetic Technologies: Theory and Practice July 24 – 29, 2016 The Insect Transformation Facility Institute for Bioscience and Biotechnology Research University of Maryland College Park Applications by May 2, 2016  (24 seats available) […]

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standard Tribolium Genome Editing Service: TriGenES.com

TriGenES, the Tribolium Genome Editing Service is a specialist in embryonic microinjections. It offers services ranging from gRNA cloning and gRNA in-vivo validation to embryonic injection of CRISPR/Cas9 as well as transposon mediated transgenesis and screening for edited animals. Based […]

Positions Available

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standard PhD position: RNAi in pest control (Germany)

The Department of Evolutionary Developmental Genetics at the Georg-August-University Göttingen (Prof. Bucher) and BAYER (Crop Science Division) are looking for a PhD Student. The position should be filled by April or May 2016. The regular working hours will be 25,87 […]

standard Post-doc Position at USDA-ARS-CMAVE, Gainesville, Florida

A Postdoctoral Research Associate position is available immediately in the Handler lab at the USDA-ARS, Center for Medical, Agricultural, and Veterinary Entomology in Gainesville, Florida. The successful candidate will be involved in a dual project involving germ-line transformation of the […]

Knowledgebase

FlyFacility, Cambridge University.  A insect genetic technologies service provider and resource

standard Fly Facility, University of Cambridge, UK

Insect Genetic Technologies Service Provider – Drosophila – Non-commercial Fly Facility, University of Cambridge, UK – http://www.flyfacility.gen.cam.ac.uk Our Drosophila microinjection service is available to fly researchers throughout the European Union. We can inject flies (including non-melanogaster Drosophila species) for P-element/piggyback […]

Service Providers

standard Insect Genetic Technologies Service Providers

Insect Genetic Technology Service Providers If you would like to add to this list use the “Make a Submission” button and select “Knowledgebase” For insects other than Drosophila melanogaster Non-Commercial The Institute for Bioscience and Biotechnology Insect Transformation Facility, University […]

CRISPRWebResources

standard CRISPR/Cas Web Resources

Web Resources for design and planning of CRISPR/Cas9 studies from Bassett and Liu (2014) CRISPR/Cas9 and Genome Editing in Drosophila. J. Genetics and Genomics 41, 7-19 doi.10.1016/j.gg.2013.12.004 Name Link Brief description Reference Drosophila CRISPR web resources OxfCRISPR (Liu Lab) http://www.oxfcrispr.org/ […]

CRISPRsDrosophila

standard CRISPR In Drosophila

Comparison of CRISPR genome engineering studies in Drosophila from Bassett and Liu (2014) CRISPR/Cas9 and Genome Editing in Drosophila. J. Genetics and Genomics 41, 7-19 doi.10.1016/j.gg.2013.12.004 Reference Gratz et al., 2013 Bassett et al., 2013 Yu et al., 2013 Kondo and Ueda, 2013 […]